Повысить шансы на успешность протокола ЭКО можно с помощью дополнительных методик вспомогательных репродуктивных технологий. Среди них – ИКСИ, ИМСИ, лазерный хетчинг и ПГТ.
ИКСИ (IntraCytoplasmic Sperm Injection)
Интрацитоплазматическая инъекция сперматозоида. Это процедура, в ходе которой один отобранный сперматозоид вводят внутрь полости яйцеклетки. Под большим увеличением (в 400 раз) эмбриолог пристально изучает сперматозоиды и подбирает для оплодотворения клетки с более высокой подвижностью и правильным строением. Далее выбранный сперматозоид вводят внутрь яйцеклетки.
ИМСИ (intracitoplasmic morphologically selected sperm injection)
Это интраплазматическая инъекция морфологически нормального сперматозоида в полость яйцеклетки. Главная разница с предыдущей процедурой в отборе клеток. При ИКСИ эмбриолог рассматривает клетки при увеличении в 400 раз, а при ИМСИ – в 6300. Такое увеличение позволяет изучить строение каждой клетки и выбрать наиболее подходящую.
Лазерный хетчинг
Это процедура, которая помогает яйцеклетке «вылупиться» из своей оболочки. У яйцеклетки есть оболочка (словно скорлупа у яйца). Именно на ней присутствуют особые рецепторы, которые помогают ее встрече со сперматозоидом. Также оболочка защищает яйцеклетку на этапе деления, но на стадии бластоцисты (5 сутки) она уже не нужна и эмбрион из нее выходит. Этот процесс и называется хетчингом. Если яйцеклетка не может самостоятельно разорвать и покинуть оболочку, ей помогают эмбриологи (лазером они делают на оболочке небольшие насечки).
ПГТ (преимплантационное генетическое тестирование)
Это генетический анализ, который позволяет исключить у будущего плода генетические и хромосомные аномалии. В этом случае эмбрионы замораживаются до получения результатов. И перенос отобранных эмбрионов происходит после получения результатов анализов.
Как долго делается ПГТ?
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Excellent article. I will be experiencing some of these issues as well..